Measurement

Part:BBa_K3380607:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-16)


No scaffold TXO ArsR regulated construct under T7 promoter (BBa_K3380103) expressing Broccoli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380607 construct can be assembled following a similar protocol (on the experience page of the BBA_K3380500 construct).

All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.

Source

The parts were purchased as de novo synthesized sequences

References